Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
3.
Detection of Mycoplasma species in raw milk
of lactating animals in Assiut and Qena city of
Egypt -
N M Saad
and K G AbdelHameed
Vet World. 2012; 5(2): 80-85
doi:
10.5455/vetworld.201
2.80-85
Abstract
The incidence of Mycoplasma Species in raw milk of
different animal species was determined. A total
of 240 random raw milk samples were collected from
cows, buffaloes, sheep and goats in Assiut and
Qena cities, Egypt (30 samples each). Fifteen
strains of Mycoplasma were isolated from raw milk.
The strains were biochemically characterized
followed by PCR assay for confirmation. On the
basis of biochemical characterization the strains
were divided into 5 species as follow M. arginini,
M. bovirhinis, M. bovis, M. species group 7, and
M. dispar. M. bovis could not be detected. The
most prevalent isolated species was M. bovirhinis
which was isolated from 6.67% of cow's milk
samples collected from Assiut City. Whereas M.
arginini, was the most prevalent species in the
examined milk samples collected from Qena City.
None of the five M. bovirhinis strains that were
identified biochemically were confirmed by PCR
assay through amplification of 16S rRNA region of
the gene that was amplified at 316 bp. Efforts
should be made to protect animals against
mycoplasma infection.
Keywords: Raw milk,
Mycoplasma bovirhinis,PCR assay.
